Protein insertion into the mitochondrial inner membrane by a twin-pore translocase.

نویسندگان

  • Peter Rehling
  • Kirstin Model
  • Katrin Brandner
  • Peter Kovermann
  • Albert Sickmann
  • Helmut E Meyer
  • Werner Kühlbrandt
  • Richard Wagner
  • Kaye N Truscott
  • Nikolaus Pfanner
چکیده

The mitochondrial inner membrane imports numerous proteins that span it multiple times using the membrane potential Deltapsi as the only external energy source. We purified the protein insertion complex (TIM22 complex), a twin-pore translocase that mediated the insertion of precursor proteins in a three-step process. After the precursor is tethered to the translocase without losing energy from the Deltapsi, two energy-requiring steps were needed. First, Deltapsi acted on the precursor protein and promoted its docking in the translocase complex. Then, Deltapsi and an internal signal peptide together induced rapid gating transitions in one pore and closing of the other pore and drove membrane insertion to completion. Thus, protein insertion was driven by the coordinated action of a twin-pore complex in two voltage-dependent steps.

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عنوان ژورنال:
  • Science

دوره 299 5613  شماره 

صفحات  -

تاریخ انتشار 2003